affychip

SymbiosisChip
Design

Format
Affymetrix Custom GeneChip (mask manufactured in 2002)
12.8 mm chip size
18 micron feature size

Bacterial Probe Sets (12058)
6270 representing ORFs & stable RNAs from 2001 S. meliloti 1021 genome annotation
Probe sets consist of thirteen oligo-(25)-mers per annotated gene

5788 representing S. meliloti intergenic sequences >150 bp
Tiling is evenly spaced on both strands
Tiling density of oligo-(25)-mers varies; the largest gap between adjacent tiled oligos is 8 bp
Large intergenic regions were equally subdivided to be <375 bp

Plant Probe Sets (9935)
Most are Medicago truncatula tentative consensus sequences (TCs)
Sequences are from TIGR database version 4
TCs were selected from root, inoculated root, nodule & pathogen-treated libraries
Chip contains some additional sequences not present in TIGR v. 4
Probe sets consist of eleven oligo-(25)-mers per TC

[SymbiosisChip home page] [SymbiosisChip publications]
[Download chip sequences] [Long lab home page] [RhizoGATE]