LOCUS       1352320       247 aa                              01-FEB-1996
DEFINITION  TRANSLATION INITIATION FACTOR IF-3 HOMOLOG DSG.
ACCESSION   1352320
PID         g1352320
DBSOURCE    SWISS-PROT: locus DSG_MYXXA, accession P48516
            class: standard.
            created: Feb 1, 1996.
            sequence updated: Feb 1, 1996.
            annotation updated: Feb 1, 1996.
            xrefs: gi: 436164, gi: 436165
KEYWORDS    INITIATION FACTOR; PROTEIN BIOSYNTHESIS.
SOURCE      Myxococcus xanthus.
  ORGANISM  Myxococcus xanthus
            Eubacteria; Proteobacteria; delta subdivision; Myxobacteria;
            Myxococcales; Myxococcaceae; Myxococcus.
REFERENCE   1  (residues 1 to 247)
  AUTHORS   Cheng,Y.L., Kalman,L.V. and Kaiser,D.
  TITLE     The dsg gene of Myxococcus xanthus encodes a protein similar to
            translation initiation factor IF3
  JOURNAL   J. Bacteriol. 176 (5), 1427-1433 (1994)
  MEDLINE   94156849
  REMARK    SEQUENCE FROM N.A., AND SEQUENCE OF 1-6.
            STRAIN=DK101
REFERENCE   2  (residues 1 to 247)
  AUTHORS   Kalman,L.V., Cheng,Y.L. and Kaiser,D.
  TITLE     The Myxococcus xanthus dsg gene product performs functions of
            translation initiation factor IF3 in vivo
  JOURNAL   J. Bacteriol. 176 (5), 1434-1442 (1994)
  MEDLINE   94156850
  REMARK    CHARACTERIZATION.
REFERENCE   3  (residues 1 to 247)
  AUTHORS   Cheng,Y. and Kaiser,D.
  TITLE     dsg, a gene required for cell-cell interaction early in Myxococcus
            development
  JOURNAL   J. Bacteriol. 171 (7), 3719-3726 (1989)
  MEDLINE   89291715
  REMARK    CHARACTERIZATION.
            STRAIN=DK101
COMMENT     [FUNCTION] IF-3 BINDS TO THE 30S RIBOSOMAL SUBUNIT AND SHIFTS THE
            EQUILIBRUM BETWEEN 70S RIBOSOMES AND THEIR 50S AND 30S SUBUNITS IN
            FAVOR OF THE FREE SUBUNITS, THUS ENHANCING THE AVAILABILITY OF 30S
            SUBUNITS ON WHICH PROTEIN SYNTHESIS INITIATION BEGINS.
            [SUBUNIT] MONOMER (BY SIMILARITY).
            [SUBCELLULAR LOCATION] CYTOPLASMIC (BY SIMILARITY).
            [SIMILARITY] TO IF-3 IN OTHER BACTERIAL SPECIES. EXCEPT FOR THE 66
            AA C-TERMINAL TAIL THAT IS NOT PRESENT IN OTHER SPECIES.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Myxococcus xanthus"
                     1..247
     Protein         1..247
                     /gene="DSG"
                     /product="TRANSLATION INITIATION FACTOR IF-3 HOMOLOG DSG"
     Site            109
                     /evidence=not_experimental
                     /note="IMPORTANT FOR 30S BINDING."
                     /site_type="other"
     Site            112
                     /evidence=not_experimental
                     /note="IMPORTANT FOR 30S BINDING."
                     /site_type="other"
     Region          134
                     /evidence=experimental
                     /note="G -> E (IN STRAINS DK429 AND DK439; CAUSES DEFECTS
                     IN FRUITING BODY DEVELOPEMENT AND IN SPORULATION)."
                     /region_name="Variant"
     Region          182..247
                     /evidence=experimental
                     /note="NEEDED FOR VEGETATIVE AND DEVELOPEMENTAL FUNCTIONS,
                     BUT NOT FOR VIABILITY."
                     /region_name="Domain"
ORIGIN      
        1 mireqrssrg gsrdqrtnrr irarevrvvg sdgsqlgvmp leaaldrart egldlveisp
       61 masppvckim dygkfkyeek kkaseakraq vtvllkevkl rpkteehdye fkvrntrrfi
      121 edgnkakvvi qfrgreithr eqgtailddv akdlkdvavv eqmprmegrl mfmilaptpk
      181 vaqkarelvr qaataakrpp ppgapgagks aagassgaee kaeetaeekk eaqaapaaae
      241 aqsptas
//