E

E

Glutamic Acid in proteins

E Value

Expectation value. The number of distinct alignments with scores equivalent to or better than the one of interest, that are expected to occur in a database search purely by chance. The lower the E-value, the more significant the score is.

echo

A UNIX command for displaying the contents of a variable to the screen (i.e. echo $TERM)

ed

A primitive UNIX text editor

electrophoresis

Separation of molecules based on their net electrical charge.

Electrostatic interactions

Ionic bonds that form between charged chemical groups

Electrostatic surface potential

The electrostatic charges on the surface of a protein. Often indicative of functional regions of a protein.

Elongation factor

A protein that plays an ancillary role in the elongation step of transcription or translation.

EMACS

A UNIX text editor.

e-mail

Electronic mail.

end labeling

the attachment of a radioactive or other label to one end of a DNA or RNA molecule

endogenous retrovirus

an active or inactive retroviral genome integrated into a host chromosome

endonuclease

an enzyme that breaks phophodiester bonds within a nucleic acid molecule

endosymbiont theory

A theory that states that the mitochondria and chloroplasts of eukaryotic cells are derived from symbiotic prokaryotes

electron density map

the distribution of electron density in a crystal (x,y,z coordinates) which is calculated from the X-ray diffraction pattern using a Fourier transform.

enhancer

A DNA sequence that binds to regulatory proteins. This binding affects the rate at which certain intervals of DNA are transcribed and hense, it affects the rate at which certain proteins are made (expressed). Often confused with "promoter", but the difference is that the enhancer sequence can be vary far away from the DNA that it regulates.

environmental variables

variables that are loaded in when you log into UNIX. Can display using the comand "setenv"

enzyme

A protein catalyst, working to accelerate specifical chemical reactions in the cell.

EOF

End of File - The character that tells the computer when the end of a computer file has been reached.

EOL

End of Line - The character that tells the computer when the end of a line of text has been reached. Different computers use different characters.

episome

A plasmid that is able to integrate into the host cell's chromosome

episome transfer

Transfer of some or all of a bacterial chromosome by integration into a plasmid

EST

Expressed Sequence Tag. A cDNA that is sequenced. An STS derived from a cDNA molecule. Thus, an EST is an STS that is found in a gene, rather than in a noncoding region of DNA. Usually short (300-500bp) single reads. They represent a snapshot of what is expressed in a given tissue, and/or at a given developmental stage.

Ethidium bromide

A type of intercalating agent that causes mutations by inserting between adjacent base pairs in a double-stranded molecule. Also used to visualize DNA under UV light.

Euchromatin

Regions of a eukaryotic chromosome that are relatively uncondensed, throught to contain active genes.

eukaryote

An organism whose DNA is enclosed in a nucleus. Includes all "higher order" life.

ex

A primitive UNIX text editor.

Excision repair

A DNA repair process that corrects various type sof DNA damage by excising and resynthesizing a region of polynucleotide.

executable file

a file of commands (usually in machine language) that can be run on a computer.

Exon

In eukaryotes, genes are often broken up into alternating regions of exons and introns. The exons contain information that is eventually represented in the mRNA.

exon-intron boundary

The nucleotide sequence at the junction between an exon and an intron.

exon skipping

aberrant splicing in which one or more exons are omitted from teh spliced RNA

exon theory of genes

An "introns early" hypothesis that holds that introns were formed when the first DNA genomes were constructed.

Exon trapping

A method, based on cloning, for identifying the positions of exons in a DNA sequence.

exonuclease

An enzyme that removes nucleotides from the ends of a nucleic acid molecule

Expressed sequence tag (EST)

A cDNA that is sequenced. An STS derived from a cDNA molecule. Thus, an EST is an STS that is found in a gene, rather than in a noncoding region of DNA. Usually short (300-500bp) single reads. They represent a snapshot of what is expressed in a given tissue, and/or at a given developmental stage.

External node

The end of a branch in a phylogenetic tree, representing one of the organisms or DNA sequences being studied.

Extrachromosomal gene

A gene in a mitochondrial or chloroplast genome

extant organism

An organism that is currently existing (not extinct)

Extreme value distribution

The probability distribution applicable to the scores of optimal local alignments.