| Gene Expression (top) |
On Campus |
Non-Profit |
Commercial |
| Expression Analysis |
(** Chip is not included **) |
Standard Sample:
Target Labeling (1ug-15ug total RNA or 0.2ug-2ug mRNA)
(>50ng using Affymetrix 3' IVT Express Protocol) |
- • Full Services: (cDNA synthesis to data acquisition; includes Bioanalyzer QC)
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| • Labeled cRNA synthesis from total RNA |
| • Hybridization and Scanning |
| • Scan Only |
• Agilent Bioanalyzer QC (per sample)
| • up to 12 samples for nano, 11 for pico |
| • each additional sample |
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| • Data Analysis only |
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Small Sample:
Target Labeling (10-100ng mRNA)
(500pg to 50ng using NuGen protocol) |
• Full Services: (cDNA synthesis to data acquisition; includes Bioanalyzer QC) |
• Labeled cRNA synthesis from total RNA |
• Hybridization and Scanning |
| • Scan Only |
• Agilent Bioanalyzer QC (per sample)
| • up to 12 samples for nano, 11 for pico |
| • each additional sample |
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• Data Analysis only |
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Gene Arrays: Full Service
Whole Transcript (WT) Sense Target Labeling to data acquisition |
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Exon Array: Full Service
Survey of alternative splicing and gene expression - exon level analysis |
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Tiling Array: Full Service
High-density tiled microarrays for transcript mapping and chromatin immunoprecipitation human, mouse, yeast |
• Only one array: |
| › Starting material: DNA |
› Starting material: RNA |
• One Set (7 arrays): |
| › Starting material: DNA |
› Starting material: RNA |
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miRNA Array: Full Service
miRNA coverage of 71 organisms, including human, mouse, rat, canine, and rhesus macaque |
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| Whole Genome DNA Analysis |
(** Chip is not included **) |
Full GeneChip mapping service
Starting material: genomic DNA |
• 10K XbaI |
• 50K Xba or Hind |
• 100K Xba and Hind |
• 250K Nsp or Sty |
• 500K Nsp and Sty (2 chips) |
• Human SNP Array 5.0 (1 chip) |
| • Human SNP Array 6.0 (1 chip) |
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Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
390 |
420 |
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Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
460 |
497 |
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Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
585 |
630 |
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| Other Services |
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| Hybridization and Scanning |
| Scan Only |
• Agilent Bioanalyzer QC (per sample)
| • up to 12 samples for nano, 11 for pico |
| • each additional sample |
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| Data Analysis only |
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On Campus |
Non-Profit |
Commercial |
| • DNA (per base): |
› 40 nmole (standard scale) |
› 200 nmole |
› 1 µmole |
› 10 µmole (set-up charge additional) |
| • RNA (per base): |
› 40 nmole |
› 200 nmole |
| • Bulk Orders (per base): |
› 20nmole scale (>24 oligos/order) |
› 96-well plate format by request (>48 oligos/order) |
| • Modifications: |
› Biotin |
› Inosine |
› Dioxyuridine |
› 5' Phosphorylation |
› 5' Thiolation |
› Internal mixed bases |
› Phosphothiolation (antisense) |
› Other modifications |
| • Purification: |
› 40 and 200 nmole scale |
› 1µmole scale |
| • LNA (per base) |
› All fees |
| **Shipping and Handling |
› USA |
› International |
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0.35 |
1 |
2 |
16 (100) |
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4 |
5 |
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0.10 |
0.10 |
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50 |
10 |
10 |
10 |
Inquire |
no charge |
no charge |
Inquire |
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7.95/oligo |
15.80/oligo |
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Inquire |
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22 |
33 |
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0.40 |
1.10 |
2.20 |
20 (120) |
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5 |
6.50 |
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0.10 |
0.10 |
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55 |
10 |
10 |
10 |
Inquire |
no charge |
no charge |
Inquire |
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7.95/oligo |
15.80/oligo |
| |
Inquire |
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22 |
33 |
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1 |
2 |
4 |
25 (150) |
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6 |
7.50 |
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0.10 |
0.10 |
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80 |
10 |
10 |
15 |
Inquire |
no charge |
no charge |
Inquire |
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7.95/oligo |
15.80/oligo |
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Inquire |
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22 |
33 |
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On Campus |
Non-Profit |
Commercial |
• Rxn not Completed (** FREE Reruns and Resequencing )
Client provides template and primer ready added (PAN will provide standard primers) |
• Rxn Clean-Up (** FREE Reruns)
Client prepares reaction and PAN cleans up reaction |
• Rxn Completed (** FREE Reruns)
Client prepares and cleans up reaction |
• 96-well plate run
Reactions not completed. |
• 96-well plate run
Client drops off plate ready to run |
• Fragment Analysis (GeneScan)
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6 |
4 |
2 |
672/plate |
195/plate |
3 |
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10 |
6 |
4 |
790/plate |
230/plate |
4.50 |
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12 |
8 |
6 |
1100/plate |
320/plate |
6 |
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On Campus |
Non-Profit |
Commercial |
• Instrument run: (per plate)
Client prepares plate ready to run |
| • Full Service: (All Prices assume that target gene primers and probes are provided by client or can be ordered from PAN's Oligo service for an additional fee) |
| •Taqman Chemistry |
| ° Taqman qPCR optimization - Primer pair per probe |
| ° Taqman qPCR, per sample |
| ° Taqman RT-qPCR, per sample |
| •SybrGreen Chemistry |
| ° SybrGreen qPCR optimization |
| ° SybrGreen qPCR, per sample |
| ° SybrGreen RT-qPCR, per sample |
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20 |
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Inquire |
Inquire |
Inquire |
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Inquire |
Inquire |
Inquire |
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25 |
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Inquire |
Inquire |
Inquire |
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Inquire |
Inquire |
Inquire |
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32 |
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Inquire |
Inquire |
Inquire |
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Inquire |
Inquire |
Inquire |
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Surface Plasmon Resonacne (top)
(Experimental set-up includes generation of biosensor surfaces for ligand capturing: Direct or indirect capture) |
On Campus |
Non-Profit |
Commercial |
| › Set-up fee (per chip, user provides chip) |
| › Experiment (full-day duration) |
| › Experiment (half-day duration) |
| › User-operated instrument session (per hour) |
| › User-operated instrument session (per day) |
| › Consulting or data analysis (half-day duration) |
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Peptide Synthesis (top)
(Non-purified peptide synthesis includes analytical HPLC and Mass spec QC) |
On Campus |
Non-Profit |
Commercial |
| • Standard scale (0.2 mmole-200 mg-500 mg)/amino acid (non purified) |
› Set-up fee (includes 5 AA) |
› Per Amino acid > 5 |
| • Immunogen scale (25 µmole-10 mg-30 mg)/amino acid (non-purified) |
› Set-up fee (includes 5 AA) |
› Per Amino acid > 5 |
| • Modification |
| • HPLC Purification |
| **Shipping and Handling |
› USA |
› International |
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183 |
35 |
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105 |
20.50 |
Inquire |
187 |
| |
22 |
33 |
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198 |
38 |
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137 |
27 |
Inquire |
213 |
| |
22 |
33 |
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286 |
54 |
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200 |
38 |
Inquire |
318 |
| |
22 |
33 |
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Protein Identification (Mass Mapping) (top) |
On Campus |
Non-Profit |
Commercial |
| Protein ID includes: In-gel digestion with trypsin; MS/MS peptide fragmentation; Mascot search against the NCBInr protein database; Report summary in pdf format. |
› Price per sample (for first 10 samples in an order) |
› Additional sample(s) (above 10 samples in the order) |
› Specialized database construction |
› Protein ID/Characterization from complex protein mixtures |
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Protein (Edman) Sequencing (top) |
On Campus |
Non-Profit |
Commercial |
| • Edman : |
› Set-up fee/sample (includes first 10 Residues) |
› Per residue (>10 residues) |
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On Campus |
Non-Profit |
Commercial |
| › Mass spectrometry by MALDI |
| › Prespotted plate |
| › Zip Tip Cleanup |
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Antibodies (top)
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On Campus |
Non-Profit |
Commercial |
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