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On Campus |
Non-Profit |
Commercial |
• DNA (per base):
| • 40 nmole (standard scale) |
| • 200 nmole |
| • 1 µmole |
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• DNA bulk orders (per base):
| • 20 nmole (>24 oligos/order) |
| • 96-well plate format by request (>48 oligos/order) |
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• RNA (per base):
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• Purification (per oligo):
| • 40 nmole and 200 nmole synthesis scales |
| • 1 umole scale |
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• Modification:
| • Biotin -- (**For any orders with 3 or more modifications/order, please inquire.) |
| • Inosine |
| • dU |
| • 5' Phosphorylation |
| • 5' Thiolation |
| • Internal mixed bases |
| • Phosphothiolation (antisense) |
| • Others |
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| • LNA (per base) |
**Shipping and Handling :
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50 |
10 |
10 |
10 |
Inquire |
no charge |
no charge |
Inquire |
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Inquire |
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55 |
10 |
10 |
10 |
Inquire |
no charge |
no charge |
Inquire |
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Inquire |
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80 |
10 |
10 |
15 |
Inquire |
no charge |
no charge |
Inquire |
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Inquire |
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| Gene Expression & Bioanalyzer (top) |
On Campus |
Non-Profit |
Commercial |
| Expression Analysis: Full Services |
(** Chip is not included **) |
3' Array:
-- cDNA synthesis to data acquisition; includes Bioanalyzer QC.
| • Standard (>50ng): using Affymetrix 3' IVT Express protocol. |
| • Small (500pg - 50ng): using NuGen protocol or PAN 2-cycle amplification protocol. |
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Gene Array:
-- Whole Transcript (WT) Sense Target Labeling to data acquisition. |
Exon Array:
-- Survey of alternative splicing and gene expression - exon level analysis. |
Tilling Array:
-- High-density tiled microarrays for transcript mapping and chromatin immunoprecipitation human, mouse, yeast.
• Only one array:
| • Starting Material: DNA |
| • Starting Material: RNA |
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• One Set (7 arrays):
| • Starting Material: DNA |
| • Starting Material: RNA |
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miRNA Array:
-- miRNA coverage of 71 organisms, including human, mouse, rat, canine, and rhesus macaque. |
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| Whole Genome DNA Analysis: Full Service |
(** Chip is not included **) |
-- Starting Material: genomic DNA
| • Human SNP Array 5.0 (1 chip) |
| • Human SNP Array 6.0 (1 chip) |
| • Cytoscan |
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| Other Services |
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| • Hybridization and Scanning |
• Agilent Bioanalyzer QC (per sample)
| • Nano or DNA 1000 (up to 12 samples), or Pico (up to 11 samples) |
| ° each additional sample |
| • Small RNA or High-Sensitivity DNA (up to 11 samples) |
| ° each additional sample |
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| • Data Analysis only |
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On Campus |
Non-Profit |
Commercial |
• Instrument run: (per plate)
-- Client prepares plate ready to run |
• Full Service: (per sample)
| • Set-up fee (per order) -- Prices do not include primers |
| • Reverse Transcription |
| • qPCR (triplicate) -- using either Taqman or SybrGreen Chemistry |
| • Custom Assay -- Taqman qPCR optimization |
| • Custom Assay -- SybrGreen qPCR optimization |
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22 |
Inquire |
15 |
10 |
Inquire |
Inquire |
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27.50 |
Inquire |
25 |
15 |
Inquire |
Inquire |
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35 |
Inquire |
28 |
18 |
Inquire |
Inquire |
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Pyrosequencing/PyroMark (top)
|
On Campus |
Non-Profit |
Commercial |
| • New Project Consultation |
| • Bisulfite Conversion (Qiagen Protocol) |
| • PCR and Pyrosequencing Run/Sample (one target region) |
| • Run Only |
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Next Generation Sequencing (top)
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On Campus |
Non-Profit |
Commercial |
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DNA Sequencing & Fragment Analysis (top) |
On Campus |
Non-Profit |
Commercial |
• DNA Sequencing:
• Per Sample Price:
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• Per Plate Price:
| • Full Service |
| • Run Only |
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• Fragment Analysis (GeneScan):
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Peptide Synthesis (top)
(Non-purified peptide synthesis includes analytical HPLC and Mass spec QC) |
On Campus |
Non-Profit |
Commercial |
• Stadard scale: (0.2 mmole-200 mg-500 mg)/amino acid (non purified)
| • Set-up fee (includes first 5 Amino Acids) |
| • Per additional Amino Acid (>5 AA) |
|
• Immunogen scale: (25 µmole-10 mg-30 mg)/amino acid (non-purified)
| • Set-up fee (includes first 5 Amino Acids) |
| • Per additional Amino Acid (>5 AA) |
|
| • HPLC Purification |
| • Modification |
**Shipping and Handling :
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On Campus |
Non-Profit |
Commercial |
| • Mass spectrometry by MALDI |
| • Prespotted plate |
| • Zip Tip Cleanup |
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On Campus |
Non-Profit |
Commercial |
• Mass Mapping: (Protein Identification)
-- In-gel digestion with trypsin; MS/MS peptide fragmentation; Mascot search against the NCBInr protein database; Report summary in pdf format.
| • Price per sample (for first 10 samples in an order) |
| • Additional sample(s) (above 10 samples in the order) |
| • Specialized database construction |
| • Protein ID/Characterization from complex protein mixtures |
|
• Edman Sequencing:
| • Set-up fee/sample (includes first 10 Residues) |
| • Per residue (>10 residues) |
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Surface Plasmon Resonance -- Biacore (top)
(Experimental set-up includes generation of biosensor surfaces for ligand capturing: Direct or indirect capture) |
On Campus |
Non-Profit |
Commercial |
| • Set-up fee (per chip, user provides chip) |
• Experiment:
| • Full-day duration |
| • Half-day duration |
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• User-operated instrument session (per hour)
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• User-operated instrument session (per day)
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| • Consulting or data analysis (half-day duration) |
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Antibody (top)
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On Campus |
Non-Profit |
Commercial |
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