The PAN Biotechnology Facility provides DNA sequencing of single stranded or double stranded DNA samples from both purified plasmids and PCR products. We accept samples in two basic formats: ones that require that PAN perform the sequencing reaction, and ones in a "reaction completed" format. Our sequencing reactions are performed using ABI Big Dye sequencing chemistry. We are presently using ABI 3100 and ABI Prism 377 sequencers. The read-lengths for the data we collect are normally between 600 and 700 bases with a basecalling accuracy > 99%. However, this is typically dependent on the quantity and quality of the templates or reactions that are submitted.