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• General Information
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We have an ABI StepOne Plus instrument for use in Quantitative PCR or Real-Time PCR.
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For Real-Time –PCR Requirements and Reagent Selection, please follow the Real-Time PCR Decision Tree.
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For more information on TaqMan Assays for SNP Genotyping, please visit ABI Website.
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• Services
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Currently the instrument is available for self-service use with the researcher providing all consumables. Please contact Shripa Patel at 650-723-3189 for more information.
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Coming Soon - Full service where investigators provide total RNA samples and the PAN Facility carries out the PCR assay and provides the investigators with a detailed report.
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• The StepOnePlus System
• The StepOnePlus System supports any real-time PCR application
- SNP Genotyping
- Gene Expression Profiling
- MicroRNA Expression
- Translocation Analysis
- Gene Detection
- Viral Load Analysis
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• Software for StepOnePlus system supports a variety of analysis methods, including:
- Absolute Quantitation
- Standard Curve
- Relative Quantitation
- Relative Standard Curve
- Comparative CT (∆∆ CT)
- Presence/absence (Plus/Minus) assays with an internal positive control
- Melt curve analysis
- Genotyping (including real-time amplification)
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