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  Genomic Services
• Oligo Synthesis
• Gene Expression
• Real-Time PCR
• Pyrosequencing
• Next Gen Sequencing
• DNA Sequencing
   & Fragment Analysis
• Single Cell Genomics
• RNA-DNA QC
  Protein Services
• Peptide Synthesis
• Mass Spectrometry
• Protein Analytics
‡ Mass Mapping
‡ Edman Sequencing
• SPR (Biacore)
 
 
Located in rooms
B065 and B017
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Gene Expression
 

SUBMISSION REQUIREMENTS

** There are different types of service provided within the Gene Expression section. Please check for the appropriate sample submission requirements for each service before placing your orders.


Full Service: (back to top)

• Sample Amount Requirements --

Array Type

Starting Material

Assay Type

Amount required for *one* sample

3’ Array - Standard
3' Array - Small

Total RNA

3’IVT Express 50-500ng
500pg - 50ng
Gene Array

Total RNA

Ambion WT Expression followed by Affymetrix GeneChip WT Terminal Labeling kit 50-500ng
Exon Array

Total RNA

Ambion WT Expression followed by Affymetrix GeneChip WT Terminal Labeling kit 50-500ng
Tiling Array

Total RNA

Affymetrix GeneChip WT ds Target or ChIP 3-5ug
miRNA Array

miRNA

GeniSphere 3DNA FlashTag Biotin HSR 100-1000ng
HTA

Total RNA

Ambion WT Expression followed by Affymetrix GeneChip WT Terminal Labeling kit 50-500ng
Human SNP

ds DNA

Affymetrix Genome-Wide Human SNP Nsp/Sty 6.0 0.5-1ug or 50ng/ul

* The assay for 3’ Array will be chosen based on the available RNA quantity.


• Sample Submission --

  • Please log in to your account (or create a new one) to place your order.
  • Choose Gene Expression section.
  • Sample Target Labeling depends on the array. Choose the appropriate labeling and proceed with the rest of the order form.
    ** The minimum amount of total RNA required is 50ngs. We prefer to have between 100ng-500ng for each sample, if possible. If you have less than 50ng, please select Small Target Labeling.  This implies a two-cycle amplification protocol. Please contact the staff in the Gene Expression team for specific information about this protocol.
  • When done, please print out the form and bring it, together with your samples, to PAN. No appointment necessary.



Hybridization and Scanning : (back to top)

• Sample Concentration Requirements --

Array Type

Hybridization Target

Target Concentration

3’ Array Biotin-labeled, fragmented cRNA 49/64 array format: 15ug in 40ul*
100 array format (Midi): 12ug in 30ul
169 array format (Mini): 7.5ug in 20ul
Gene 1.0ST Array Biotin-labeled, fragmented ss DNA ~27ul**
Exon Array Biotin-labeled, fragmented ss DNA ~60ul**
Tiling Array Biotin-labeled, fragmented ds DNA ~7.5ug (~60ul)***
miRNA Array GeniSphere 3DNA FlashTag Biotin HSR 21ul
Human SNP 6.0 Array Biotin-labeled, fragmented ds DNA ~73ul

* If using Affymetrix 3’IVT Express protocol
** If using Affymetrix GeneChip WT Terminal Labeling protocol
***If using Affymetrix GeneChip WT Double-Stranded Target protocol