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Edman Sequencing
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Protein Analytics - Edman Sequencing
 

FREQUENTLY ASKED QUESTIONS
  1. Turn-around time?
  2. In what form should samples be submitted?
  3. How much sample do you need for the analysis?


  1. Turn-around time? (top)
    One week for samples submitted by members of the Stanford community; two weeks or longer for off campus clients.

  2. In what form should samples be submitted? (top)
    Best is adsorbed to PVDF membrane, usually following blotting from an SDS-PAGE gel.  Only PVDF can be used.  Nitrocellulose, for example, is absolutely forbidden.  Alternatively, protein/peptides can be submitted as a dry powder, such as synthetic peptides, or in solution.  Unless adsorbed to PVDF membrane, you must provide us with a complete list of ALL components of your sample (buffers, solvents, solutes, etc.) before we will undertake it’s analysis.

  3. How much sample do you need for the analysis? (top)
    Blotted samples should contain 100 pmol of protein.  Proteins or peptides dried or in solution may be identified at a level as low as 5 pmol.