The PAN Biotechnology Facility offers several services for identifying proteins and for determining primary sequence information for proteins and peptides.

• Mass Mapping is a convenient and sensitive method for determining the identity of proteins. Mass mapping involves tryptic digestion of proteins and mass analysis of the resulting mixture of peptides.  As long as a protein’s sequence is present in a database, it can be accurately identified by this method.  Samples can be submitted in gels (preferred), in solution, or dried. Mass Mapping is performed using the 4700 Proteomics Analyzer.

• LCQ Mass Spec can be employed similarly to Mass Mapping except that it permits identification of mixtures of proteins up to about about a dozen or so in a single sample.  Samples can be submitted in gels, in solution, or dried.

• Edman Sequencing provides primary sequence information by automated analysis of sequential amino acid residues.   Samples can be submitted immobilized on PVDF membranes (preferred), in solution or dried samples. For Stanford clients, we can extend the method  to include protease digestion, separation by HPLC and subsequent Edman sequencing of peptides.