The PAN Biotechnology Facility offers several services for identifying proteins and for determining primary sequence information for proteins and peptides.

• Mass Mapping is a convenient and sensitive method for determining the identity of proteins. Mass mapping involves tryptic digestion of proteins and mass analysis of the resulting mixture of peptides.  As long as a protein’s sequence is present in a database, it can be accurately identified by this method.  Samples can be submitted in gels (preferred), in solution, or dried. Mass Mapping is performed using the 4700 Proteomics Analyzer.

• LCQ Mass Spec can be employed similarly to Mass Mapping except that it permits identification of mixtures of proteins up to about about a dozen or so in a single sample.  Samples can be submitted in gels, in solution, or dried.