INSTRUMENT
Qiagen PyroMark Q24 - Pyrosequencing  technology for real-time, sequence-based detection for quantification of sequence variants (SNPs/mutation detection) )and epigenetic methylation (CpG analysis).

DESCRIPTION • Pyrosequencing is a simple and robust technique that sequences by synthesis. Using a short PCR product as a template, bases are added systematically and incorporated into a replicating DNA strand. Pyrosequencing utilizes a series of enzymatic reactions to detect pyrophosphate that is released during base incorporation and uses the detection data to establish the sequence of the template strand.

Click here for animation of this process

• The data is a quantitative measure of each detected nucleotide and is ideal for measuring the relative amounts of alleles. This property allows the quantification of DNA methylation, heterozygosity, ploidy levels, multi-copy genes, pooled DNA samples, hematopoeitic chimerism, and mixed genotypes in heterogeneous samples (e.g. tumor and normal cells).

Service Options
Our service can be tailored to individual requirements and may include all or a combination of the following:

• Assay design
• Oligonucleotide synthesis
• Assay optimization
• Sample assay
• Interpretation and troubleshooting of results

Please contact us to discuss your individual requirements.

Contact:   Shripa Patel
Phone: (650) 723-3189
Email: shripa@stanford.edu