• General Information

We have an ABI StepOnePlus instrument for use in Quantitative PCR or Real-Time PCR. For Real-Time –PCR Requirements and Reagent Selection, please follow the Real-Time PCR Decision Tree. For more information on TaqMan Assays for SNP Genotyping, please visit ABI Website.

• Services

Currently the instrument is available for self-service use with the researcher providing all consumables. You need to make a reservation before using the machine by signing up at this site. Please contact Shripa Patel at 650-723-3189 for more information. . Full service: Investigators provide total RNA samples and the PAN Facility carries out the PCR assay and provides the investigators with a detailed report. Please contact Shripa Patel at 650-723-3189 for more information.

• The StepOnePlus System

• The StepOnePlus System supports any real-time PCR application SNP Genotyping Gene Expression Profiling MicroRNA Expression Translocation Analysis Gene Detection Viral Load Analysis • Software for StepOnePlus system supports a variety of analysis methods, including: Absolute Quantitation Standard Curve Relative Quantitation Relative Standard Curve Comparative CT (∆∆ CT) Presence/absence (Plus/Minus) assays with an internal positive control Melt curve analysis Genotyping (including real-time amplification)