Timecourse Data: Metabolic Chart

Distinct temporal patterns of induction or repression help to group genes that share regulatory properties. (A) Temporal profile of the cell density, as measured by optical density at 600nm, and glucose concentration in the media. (B) Seven genes exhibited a strong induction (>9 fold) only at the last time point. (20.5 hours). With the exception of IDP2, each of these genes has a carbon source response element (CSRE) UAS. There were no additional genes observed to match this profile. (C) Seven members of a class of genes marked by early induction with a peak in mRNA levels at 18.5 hours. Each of these genes contain stress response element (STRE) motif repeats in their upstream promoter regions. (D) Cytochrome-c oxidase and ubiquinol cytochrome-c reductase genes. Marked by an induction coincident with the diauxic shift, each of these genes contain a consensus binding motif for the HAP2,3,4 protein complex. At least 17 genes shared a similar expression profile. (E) SAM1, GPP1, and several genes of unknown function are repressed prior to the diauxic shift, and continue to be repressed upon entry into stationary phase. (F) Ribosomal protein genes comprise a large class of genes which are repressed upon depletion of glucose. Each of the genes profiled here contains one or more RAP1 binding motif upstream of its promoter. RAP1 is a transcriptional regulator of most ribosomal proteins.

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