Post-Processing of Oligo Arrays on Corning Ultra GAPS slides

Martin Buess

DRAFT VERSION 01.17.06

USE THIS FOR CORNING ULTRA GAPS SLIDES ONLY. DO NOT USE ON POLYLYSINE COATED SURFACES.

Postprocessing slides

  1. Using a diamond scribe, etch the non-printed side of the array to denote the boundaries of the printed area.
  2. UV cross-link printed DNA onto glass substrate with 65mJ of energy in a Stratalinker (650 x 100uJ) and transfer arrays on a slide rack.
  3. Prehybridization in 5x SSC, 0.1 mg/ml BSA, 0.1% SDS at 42°C for 60 Min. (300 ml MQ H2O, 100 ml filtered 20 x SSC, 4 ml 10mg/ml BSA stock solution (filtered), 4 ml 10% SDS, prewarm in 42°C waterbath for 60 min)
  4. Rinse in arrays in 0.1 x SSC for 5 min at RT
  5. Repeat step 4
  6. Transfer arrays to MQ water and incubate at RT for 30 sec.
  7. Spin dry arrays in a table to centrifuge at 500 rpm for 5 min.
NOTE: Use arrays the same day that they are postprocessed