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Figure 5. Genes induced during sporulation.

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(A) The seven average expression profiles (Fig. 4B), arranged by time of first induction, were used to further classify the induced genes shown in Fig. 4A, by first assigning each gene to the expression class with which it had the highest correlation. Genes assigned to each class were then ordered with respect to correlation with adjacent temporal classes: genes were sorted within each class based on the difference in the correlation coefficients with neighboring classes. The black and white gradients for each temporal class on the left edge of the figure represents a positional reference: the border regions between any two classes are lighter, and the middle of the class is dark. Genes within a class that also closely correlate with a neighboring class are likely to be in the lighter regions, whereas genes that are less likely to correlate with neighboring classes are likely to be in dark regions of the gradient. In this graphical display, changes in mRNA level resulting from ectopic expression of Ndt80 in vegetative cells are shown in the eighth column (Gal-Ndt80). Two sets of columns with blue bars identify genes whose promoters contain a putative URS1 or MSE regulatory sequence as labeled. The degree to which the sequence matches the consensus for each of these regulatory elements is indicated by the brightness of the bar - the best matches are represented by bright blue bars on the left, and less stringent matches by dark blue bars on the right. The most stringent match for the URS1 site was 5'TCGGCGGCTDW, and the least stringent was 5'GGCGGC. The most stringent match for the MSE site was 5'HDVKNCACAAAAD, and the least stringent was 5'DNCRCAAAWD.