Biochemistry 8: 3214-8 (1969)[69285371]
When native nucleohistone is treated with formaldehyde, the
resulting soluble complex does not dissociate in solutions of high
ionic strength and may be banded in a CsCI gradient. The buoyant
density of the complex, 1.411 g/cc, is that calculated from the ratio
of deoxyribonucleic acid to histone in nucleohistone. Formaldehyde
treatment of nucleohistone cross-links histone molecules to
deoxyribonucleic acid. Thus the histone of the formaldehyde-treated
complex is no longer soluble in acid. Treatment with pronase removes
96% of the protein from deoxyribonucleic acid, leaving 4% firmly
bound, an amount which is in approximate stoichiometric proportion to
the formaldehyde cross-links between deoxyribonucleic acid and
protein.
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