Cell 15: 733-42 (1978)[79084143]
A method for purifying sequences adjacent to satellite DNA in the
heterochromatin of D. melanogaster is described. A cloned DNA segment
containing part of a copia gene adjacent to 1.688 g/cm3 satellite DNA
has been isolated. The copia genes compose a repeated gene family
which codes for abundant cytoplasmic poly(a)-containing RNA (Young
and Hogness, 1977; Finnegan et al., 1978). We have identified two
major poly (A)-containing RNA species [5.2 and 2.1 kilobases
(kb)] produced by the copia gene family. The cloned segment
contains copia sequences homologous to the 5' end of RNA within 0.65
kb of the 1.688 satellite DNA sequences. Seven different cloned copia
genes from elsewhere in the genome have also been isolated, and a 5.2
kb region present in five of the clones was identified as copia by
heteroduplex analysis. In addition, three ususual copies of copia
were found: a "partial" copy of the gene (3.7 kb) which has one
endpoint in common with the 5.2 kb unit; a copia gene flanked on one
side by a 1.6 kb sequence and on the other by the same 1.6 kb
sequence in the inverted orientation; and a copia gene flanked only
on one side by the same sequence.
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