Proc Natl Acad Sci U S A 76: 726-30 (1979)[79137183]
Using a nitrocellulose filter binding assay, we have detected and
partially purified a protein from embryos of Drosophila melanogaster
that preferentially binds to a highly repeated satellite DNA of the
same species. Formation of the satellite DNA-protein complex requires
physiological conditions of salt and temperature, but once formed,
the complex is stable in high salt (1 M NaCl) or at low temperature.
Optimal formation of the specific complex also requires the satellite
DNA to be in a supertwisted conformation. The protein interacts with
a limited region within the 359-base-pair repeated sequence of the
satellite DNA.
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