Biochemistry 7: 3149-55 (1968)[69013573]
The nonhistone protein of interphase chromatin of rat liver has been
isolated, using sodium dodecylsulfate, and partially characterized.
The chromosomal nonhistone protein is rich in acidic amino acids and
contains tryptophan. The physical state of chromosomal nonhistone
protein is markedly dependent upon detergent. In the presence of
detergent (0.1 %) the nonhistone protein gives S(20,W) of 2.7 S and
an average molecular weight of 14,300. On removal or lowering of
detergent concentrations the protein aggregates. The chromosomal
nonhistone protein interacts with histones to form an insoluble
complex under appropriate conditions. A chromosomal nonhistone
protein-deoxyribonucleic acid complex can he made by a NaCI gradient
dialysis of mixtures of deoxyribonucleic acid and the nonhistone
protein. Such a complex shows the same activity in support of
ribonucleic acid synthesis as does pure deoxyribonucleic acid.
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