Global Analysis of Gene Expression in the Dauer Larvae of Caenorhabditis elegans

John Wang ¹ and Stuart K. Kim^1,2,3

¹Department of Developmental Biology
Stanford University Medical Center
Stanford, CA 94305

²Department of Genetics
Stanford University Medical Center
Stanford, CA 94305

³corresponding author
650-725-7671
650-725-7739
kim@cmgm.stanford.edu

Figure 4. Dauer expression of glycolysis and gluconeogenesis genes.

The glycolytic and gluconeogenic pathways are up-regulated in dauers as inferred from changes in gene expression. None of the C. elegans genes encoding the enzymes that catalyze each step in these two pathways has been explicitly defined, but the ORFs illustrated show significant homology to the respective enzymes. Red lettering and boxes refer to genes that are dauer-enriched (p<0.01) when comparing adjustment dauer at 0 hours to adjustment dauer at 12 hours. Correspondingly, red arrows indicate inferred high levels of enzyme activity in dauers. Black lettering in gray boxes indicates no significant difference in expression. The magnitude of induction or repression for each gene is indicated. Lines that end in filled circles connect genes with the enzymatic step. Some key metabolic intermediates are shown. Glucose-6-phosphatase activity (N4) is depicted as a dashed line because there is no clear homolog that encodes that activity. F47B8.10 encodes a glucose-6-phosphate translocase, which functions in a complex with the phosphatase in vertebrates.

Glycolysis enzymes: E1 = hexokinase; E2 = glucose-6-phosphate isomerase; E3 = phosphofructokinase; E4 = fructose-bisphosphate aldolase; E5 = triose phosphate isomerase; E6 = glyceraldehyde-3-phosphate dehydrogenase; E7 = phosphoglycerate kinase; E8 = phosphoglycerate mutase; E9 = enolase; E10 = pyruvate kinase.

Gluconeogenic enzymes: N1 = pyruvate carboxylase; N2 = phosphenolpyruvate carboxykinase; N3 = fructose-1,6-bisphosphatase; N4 = glucose-6-phosphatase.

Glu-6-P = glucose-6-phosphate; Fru-6-P = fructose-6-phosphate; Fru-1,6-BP = fructose-1,6-bisphosphate; DHAP = dihydroxyacetone phosphate; G3P = glyceraldehyde-3-phosphate; PEP = phosphoenolpyruvate.

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