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Global Analysis of Gene Expression in the Dauer Larvae of Caenorhabditis elegans

John Wang 1 and Stuart K. Kim1,2,3


1Department of Developmental Biology
Stanford University Medical Center
Stanford, CA 94305

2Department of Genetics
Stanford University Medical Center
Stanford, CA 94305

3corresponding author
650-725-7671
650-725-7739
kim@cmgm.stanford.edu

Chip and SOM picture



Supplemental Figure 2

Supplemental Figure 2

Supplemental Figure 2. Expression patterns of genes in mount 22.

Hierarchical clustering was used to display the ratio of dauer to L1 at 0 hours (D/L1) and the expression ratios of the 154 mount 22 genes during the dauer exit and L1 starvation time courses. In general the expression patterns for the genes in mount 22 are down regulated during dauer exit as seen by the pattern of increasing blue intensity during the time course. This would suggest that the mount 22 genes are dauer-enriched and is also reflected by the fact that mount 22 is over-represented in the dauer- enriched gene class. However, the majority of mount 22 gene expression patterns appear to be derived from contaminating carcasses. First, most of the genes in mount 22 are expressed less in dauers than in mixed-staged reference RNA as indicated in column 3 (mostly blue). Second, data from the pure dauer population indicates that most genes are not significantly more expressed at 0 h than at 12 h after feeding of the pure dauers (column 2, few strong yellow). Rows represent different genes and columns represent different times during the time course. Scale shows level of expression. (D) is dauer and (L1) is L1 larvae.
Mount 5 Cluster

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 Last updated March 24, 2003