Kim lab C. elegans microarrays


Publications 

The C. elegans microarray center will be closing as of June 2004. We can accept samples until then for anyone. After June, you should contact Stuart Kim to discuss possible ways to collaborate to do DNA microarray experiments (kim@cmgm.stanford.edu).

previous message: Our general goal is to develop C. elegans microarray technology, and to make this technology available to all other C. elegans labs. We are collaborating with any C. elegans lab by hybridizing their RNA samples to C. elegans microarrays, and we are also helping to provide needed tools and reagents so that other labs can establish their own microarraying facilities. Any academic lab will have free access to these microarrays. RNA will be prepared in each of the individual labs, microarray experiments will performed at Stanford and results will be reported over the web.

The full genome chips have been printed (17,815 genes, 94% of the genome) and are now being used for experiments. These microarrays contain a spot for each gene, plus some control spots.

If you are interested using the Kim lab microarrays, please email Min Jiang (mjiang@cmgm.stanford.edu).

Once we do an experiment for you, you need to register to get a password for the database. Then you can see your results on the Stanford Microarray Database.

As of April, 2004, we have done about 1200 experiments with about 100 labs.

General information about C. elegans microarrays.

How to grow worms to make RNA.

Tips on how to analyze your data.

Coding key for early experiments using the 11,990 spot chips. This information also can be found in the Stanford Microarray Database. (password is needed).


Description of the worm microarray

Click here for list of all primer sequences for DNA on the full genome chip

The primer list can also be searched.


 

This work has been funded by grants from the National Center for Research Resources, the Merck Genome Research Institute and Rhone-Poulenc/Rorer Gencell.


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Please send comments or questions regarding this home page to Jim Lund (jiml@stanford.edu)