As a graduate student at Harvard and a predoctoral fellow of the Howard Hughes Medical Institute, I developed a transgenic method in Drosophila by which any two genes can be integrated into the same location in the genome. The method, called transgene coplacement, is described in this paper (also click here for Cre/loxP vector and stock information).
I used transgene coplacement of two divergent alcohol dehydrogenase homologues to explore the nature of genomic position effects on gene expression, and to validate the method as a tool for evolutionary studies of regulatory divergence. The research is described in this paper (this is a link to the PNAS web site).
I used my transgenic lines to explore the effect of Adh genotype on the oviposition behavior of females given a choice between normal medium and medium supplemented with ethanol. The research is described in this paper.
I reviewed the transgene coplacement method and the strains and vectors used in its application in this paper.
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