LOCUS XXU13850 4948 bp DNA circular SYN 13-DEC-1994 DEFINITION pGEX-2T cloning vector, complete sequence. ACCESSION U13850 NID g595709 KEYWORDS glutathione S-transferase; beta-lactamase; lac repressor. SOURCE unidentified cloning vector. ORGANISM unidentified cloning vector artificial sequence; cloning vectors. REFERENCE 1 (bases 1 to 4948) AUTHORS Malone,J.A. TITLE pGEX-2T: A cloning vector for the inducible expression of genes as glutathione S-transferase fusion proteins containing a thrombin cleavage site JOURNAL Unpublished (1994) REFERENCE 2 (bases 1 to 4948) AUTHORS Smith,D.B. and Johnson,K.S. TITLE Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase JOURNAL Gene 67 (1), 31-40 (1988) MEDLINE 88329742 REFERENCE 3 (bases 264 to 911) AUTHORS Smith,D.B., Davern,K.M., Board,P.G., Tiu,W.U., Garcia,E.G. and Mitchell,G.F. TITLE Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase [published erratum appears in Proc Natl Acad Sci U S A 1987 Sep;84(18):6541] JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83 (22), 8703-8707 (1986) MEDLINE 87041520 REFERENCE 4 (bases 881 to 911) AUTHORS Smith,D.B., Davern,K.M., Board,P.G., Tiu,W.U., Garcia,E.G. and Mitchell,G.F. TITLE Correction: Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase JOURNAL Proc. Natl. Acad. Sci. U.S.A. 84, 6541-6541 (1987) REFERENCE 5 (bases 1 to 4948) AUTHORS Malone,J.A. TITLE Direct Submission JOURNAL Submitted (19-AUG-1994) James A. Malone, International Technical Services, Molecular Biology Reagents Division, Pharmacia Biotech Inc., 2202 N. Bartlett Ave., Milwaukee, WI 53202-1009, USA FEATURES Location/Qualifiers source 1. .4948 /organism="unidentified cloning vector" /lab_host="Escherichia coli" promoter 183. .211 /standard_name="tac" /note="tac promoter for inducible expression of glutathione S-transferase" protein_bind 217. .237 /gene="lac" /bound_moiety="lac repressor protein" CDS 258. .956 /citation=[3] /citation=[4] /codon_start=1 /transl_table=11 /product="glutathione S-transferase" /db_xref="PID:g595710" /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK SDLVPRGSPGIHRD" misc_feature 918. .935 /note="encodes thrombin recognition site" misc_feature 930. .945 /note="Multiple Cloning Site (MCS); contains the unique restriction sites BamHI, SmaI and EcoRI" promoter 1286. .1314 /gene="bla" CDS 1356. .2216 /gene="bla" /codon_start=1 /transl_table=11 /product="beta-lactamase" /db_xref="PID:g595711" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2974 /note="base 2974 represents the first base of the newly synthesized single strand" /direction=right CDS 3297. .4379 /gene="lacIq" /codon_start=1 /transl_table=11 /product="lac repressor" /db_xref="PID:g595712" /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" BASE COUNT 1223 a 1193 c 1284 g 1248 t ORIGIN U13850 Length: 4948 September 3, 1996 16:06 Type: N Check: 4968 .. 1 ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC 51 GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT 101 CGCTCAAGGC GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT 151 AACGGTTCTG GCAAATATTC TGAAATGAGC TGTTGACAAT TAATCATCGG 201 CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 251 AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC 301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT 351 TTGTATGAGC GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT 401 GGGTTTGGAG TTTCCCAATC TTCCTTATTA TATTGATGGT GATGTTAAAT 451 TAACACAGTC TATGGCCATC ATACGTTATA TAGCTGACAA GCACAACATG 501 TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC TTGAAGGAGC 551 GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT 601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA 651 ATGTTCGAAG ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT 701 AACCCATCCT GACTTCATGT TGTATGACGC TCTTGATGTT GTTTTATACA 751 TGGACCCAAT GTGCCTGGAT GCGTTCCCAA AATTAGTTTG TTTTAAAAAA 801 CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT CCAGCAAGTA 851 TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC 901 ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCCCGGG AATTCATCGT 951 GACTGACTGA CGATCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT 1001 CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG 1051 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT 1101 CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATAATTC 1151 TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA 1201 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG 1251 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 1301 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA 1351 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG 1401 GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA 1451 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC 1501 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA 1551 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT 1601 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG 1651 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG 1701 ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC 1751 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT 1801 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG 1851 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC 1901 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC 1951 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA 2001 GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA 2051 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC 2101 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG 2151 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 2201 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA 2251 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT 2301 TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG 2351 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT 2401 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG 2451 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 2501 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT 2551 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT 2601 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 2651 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG 2701 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC 2751 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 2801 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG 2851 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT 2901 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 2951 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC 3001 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA 3051 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 3101 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG 3151 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT 3201 TCACACCGCA TAAATTCCGA CACCATCGAA TGGTGCAAAA CCTTTCGCGG 3251 TATGGCATGA TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG GTGAATGTGA 3301 AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG 3351 ACCGTTTCCC GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG 3401 GGAAAAAGTG GAAGCGGCGA TGGCGGAGCT GAATTACATT CCCAACCGCG 3451 TGGCACAACA ACTGGCGGGC AAACAGTCGT TGCTGATTGG CGTTGCCACC 3501 TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG CGATTAAATC 3551 TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA 3601 GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC 3651 GTCAGTGGGC TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC 3701 TGTGGAAGCT GCCTGCACTA ATGTTCCGGC GTTATTTCTT GATGTCTCTG 3751 ACCAGACACC CATCAACAGT ATTATTTTCT CCCATGAAGA CGGTACGCGA 3801 CTGGGCGTGG AGCATCTGGT CGCATTGGGT CACCAGCAAA TCGCGCTGTT 3851 AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG GCTGGCTGGC 3901 ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA ACGGGAAGGC 3951 GACTGGAGTG CCATGTCCGG TTTTCAACAA ACCATGCAAA TGCTGAATGA 4001 GGGCATCGTT CCCACTGCGA TGCTGGTTGC CAACGATCAG ATGGCGCTGG 4051 GCGCAATGCG CGCCATTACC GAGTCCGGGC TGCGCGTTGG TGCGGATATC 4101 TCGGTAGTGG GATACGACGA TACCGAAGAC AGCTCATGTT ATATCCCGCC 4151 GTTAACCACC ATCAAACAGG ATTTTCGCCT GCTGGGGCAA ACCAGCGTGG 4201 ACCGCTTGCT GCAACTCTCT CAGGGCCAGG CGGTGAAGGG CAATCAGCTG 4251 TTGCCCGTCT CACTGGTGAA AAGAAAAACC ACCCTGGCGC CCAATACGCA 4301 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC 4351 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG 4401 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC 4451 GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 4501 TATGACCATG ATTACGGATT CACTGGCCGT CGTTTTACAA CGTCGTGACT 4551 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT 4601 TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA 4651 ACAGTTGCGC AGCCTGAATG GCGAATGGCG CTTTGCCTGG TTTCCGGCAC 4701 CAGAAGCGGT GCCGGAAAGC TGGCTGGAGT GCGATCTTCC TGAGGCCGAT 4751 ACTGTCGTCG TCCCCTCAAA CTGGCAGATG CACGGTTACG ATGCGCCCAT 4801 CTACACCAAC GTAACCTATC CCATTACGGT CAATCCGCCG TTTGTTCCCA 4851 CGGAGAATCC GACGGGTTGT TACTCGCTCA CATTTAATGT TGATGAAAGC 4901 TGGCTACAGG AAGGCCAGAC GCGAATTATT TTTGATGGCG TTGGAATT