Welcome to the Home of Vesicle Trafficking
A small repository of synaptic protein information
- Syntaxin 1A - Rattus norvegicus - Accession # M95734
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Genomic clone in pRC-CMV with the T7 promoter 5' to the clone.
PCR fragment including start ATG subcloned into NotI;
linearize with XbaI.
- Syntaxin 1A-1 in pGEX-KG includes amino acids 8-288.
- Syntaxin 1A-3 in pGEX-KG includes amino acids 4-288.
- Syntaxin 1A-4 in pGEX-KG includes amino acids 8-76.
- Syntaxin 1A-5 in pGEX-KG includes amino acids 4-114.
- Syntaxin 1A-6 in pGEX-KG includes amino acids 4-193.
- Syntaxin 1A-7 in pGEX-KG includes amino acids 77-288.
- Syntaxin 1A-8 in pGEX-KG includes amino acids 115-288.
- Syntaxin 1A-9 in pGEX-KG includes amino acids 194-288.
- Syntaxin 1A-11 in pGEX-KG includes amino acids 4-267.
- Syntaxin 1A-12 in pGEX-KG includes amino acids 221-288.
- Syntaxin 1A-14 in pGEX-KG includes amino acids 77-267.
- Syntaxin 1A-15 in pGEX-KG includes amino acids 115-267.
- Syntaxin 1A-16 in pGEX-KG includes amino acids 191-267.
- Syntaxin 1B - Rattus norvegicus - Accession # M95735
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Genomic clone in Bluescript SKII- EcoRI with T7 promoter 5' to the clone.
Includes some UTR on both sides. However, most of the untranslated
region shown in the M95735 map is deleted in this clone.
- Syntaxin 2 - Rattus norvegicus - Accession # L20823
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Genomic clone in Bluescript SKII- EcoRI with the T3 promoter 5' to the clone.
The L20823 map doesn't include UTR (sequence unavailable.)
Cleavage with EcoRI yields two 3-kb fragments, vector and clone,
which look like a single band on agarose gels. Digestion with
BamHI/EcoRI yields three bands: 3.0, 2.3, 0.7 kB.
- Syntaxin 2' - Rattus norvegicus - Accession # L20889
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Genomic clone in Bluescript SKII- EcoRI with the T3 promoter 5' to the clone.
The L20823 map doesn't include UTR (sequence unavailable.)
Cleavage with EcoRI yields two 3-kb fragments, vector and clone,
which look like a single band on agarose gels. Digestion with
BamHI/EcoRI yields three bands: 3.0, 2.3, 0.7 kB.
- Syntaxin 2'' - Rattus norvegicus - Accession #
L20888
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Genomic clone in Bluescript SKII-.
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Syntaxin 2'' 1-2 in pGEX-KG includes amino acids 4-191 (plus aa 265-267???)
Subcloned as a PCR fragment with EcoRI/HindIII linkers.
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Syntaxin 2'' 1-4 in pGEX-KG includes amino acids 4-267.
Subcloned as a PCR fragment with EcoRI/HindIII linkers.
-
Syntaxin 2'' 3-4 in pGEX-KG includes amino acids 192-267.
Subcloned as a PCR fragment with EcoRI/HindIII linkers.
- For all of these PGEX constructs, if you have any questions about their construction,
please consult the following reference: "Protein-Protein Interactions Contributing to
the Specificity of Intracellular Vesicular Trafficking" N Calakos, MK Bennett, KE
Peterson, RH Scheller, SCIENCE, 25 February 1994, Volume 263, pp. 1146-1149.
- Syntaxin 3 - Rattus norvegicus - Accession # L20820
-
Genomic clone in Bluescript SKII- EcoRI with the T3 promoter 5' to the clone.
Cleavage with EcoRI yields a doublet at 3 kB; digestion with SacI/EcoRI
gives 3 kB vector and 2.0 and 0.7 kB insert fragments.
-
Syntaxin 3 1-2 in pGEX-KG includes amino acids 4-187.
Subcloned as a PCR fragment with EcoRI/HindIII linkers.
-
Syntaxin 3 1-4 in pGEX-KG includes amino acids 4-264.
Subcloned as a PCR fragment with EcoRI/HindIII linkers.
-
Syntaxin 3 3-4 in pGEX-KG includes amino acids 188-264.
- For all of these PGEX constructs, if you have any questions about their construction,
please consult the following reference: "Protein-Protein Interactions Contributing to
the Specificity of Intracellular Vesicular Trafficking" N Calakos, MK Bennett, KE
Peterson, RH Scheller, SCIENCE, 25 February 1994, Volume 263, pp. 1146-1149.
Subcloned as a PCR fragment with EcoRI/HindIII linkers.
- Syntaxin 4 - Rattus norvegicus - Accession # L20821
-
Genomic clone in Bluescript SKII- EcoRI with T7 promoter 5' to the clone.
The L20821 map doesn't include UTR (sequence unavailable.)
-
Syntaxin 4 1-2 in pGEX-KG includes amino acids 5-196.
Subcloned as a PCR fragment with BamHI/HindIII linkers.
-
Syntaxin 4 1-4 in pGEX-KG includes amino acids 5-274.
Subcloned as a PCR fragment with BamHI/HindIII linkers.
-
Syntaxin 4 3-4 in pGEX-KG includes amino acids 197-274.
Subcloned as a PCR fragment with BamHI/HindIII linkers.
- For all of these PGEX constructs, if you have any questions about their construction,
please consult the following reference: "Protein-Protein Interactions Contributing to
the Specificity of Intracellular Vesicular Trafficking" N Calakos, MK Bennett, KE
Peterson, RH Scheller, SCIENCE, 25 February 1994, Volume 263, pp. 1146-1149.
- Syntaxin 5 - Rattus norvegicus - Accession # L20822
-
Genomic clone in Bluescript SKII- EcoRI with the T3 promoter 5' to the clone.
- Syntaxin 6 - Rattus norvegicus - Accession # XXXXXX
