Welcome to the Home of Vesicle Trafficking
A small repository of synaptic protein information
- VAMP 1 - Rattus norvegicus - Accession # M24104
-
Genomic clone - 1.55 kB insert in Bluescript KSII EcoRI
with the T3 promoter 5' to the clone. Contains UTR that was
not sequenced.
-
VAMP 1 in pGEX-KG - amino acids 1-96, as a 300bp PCR fragment, subcloned into EcoRI/HindIII.
If you have any questions about this construct, please consult the following
reference: "Protein-Protein Interactions Contributing to the Specificity of Intracellular
Vesicular Trafficking" N Calakos, MK Bennett, KE Peterson, RH Scheller, SCIENCE,
25 February 1994, Volume 263, pp. 1146-1149.
- VAMP 2 - Rattus norvegicus - Accession # M24105
-
Genomic clone - 2.0 kB insert in Bluescript KSII+ EcoRI with the
T3 promoter 5' to the clone
-or-
0.9 kB insert in Bluescript KSII+ EcoRI (most of the 3' UTR removed)
with the T3 promoter 5' to the clone.
-
VAMP 2 in pGEX-KG - amino acids 1-94 subcloned using PCR into the EcoRI/HindIII site of
pGEX-KG. with with NcoI linkers. The recombinant protein contains the sequence
"GSPGISGGGGGILDSMAELDTPA" at the amino terminus after thrombin cleavage.
If you have any questions about this construct, please consult the following
reference: "Protein-Protein Interactions Contributing to the Specificity of Intracellular
Vesicular Trafficking" N Calakos, MK Bennett, KE Peterson, RH Scheller, SCIENCE,
25 February 1994, Volume 263, pp. 1146-1149.
-
FLAG-VAMP 2 in pRC-CMV - subcloned with NotI, T7 promoter 5' to the
clone.
