Welcome to the
Home
of Vesicle Trafficking
A small repository of synaptic protein information
Caution! In converting these protocols to HTML, some units have been scrambled.
Specifically, microliters (ul) often appear as milliliters (ml).
Think first!
Affinity Purification of Antibodies
CIP Dephosphorylation of Linearized DNA
Colony Hybridization
Double-Stranded DNA Sequencing
Drosophila Head Collection
Drosophila DNA Prep
Drosophila RNA Prep
Exo/S1 Deletion Series
Genomic DNA from Tissues
Genomic Quick Prep
GST Fusion Protein Prep
Helper Phage Prep
Immunohistochemistry on Whole-Mount Embryos
Immunohistochemistry on Drosophila Brains
Immunohistochemistry on Schneider Line 2 Cells
In Situ Hybridization to Tissue Sections
(Long Protocol)
Plasmid Prep: Alkaline Lysis
Plasmid Prep: Large Scale
Transformation of E. coli by Electroporation