Welcome to the Home of Vesicle Trafficking
A small repository of synaptic protein information
Caution! In converting these protocols to HTML, some units have been scrambled.
Specifically, microliters (ul) often appear as milliliters (ml).
Caution! In converting these protocols to HTML, some units have been scrambled.
1. Freeze-thaw cells 3X in dry ice-EtOH bath (heat to 37oC after freezing).
2. Add 0.5ml Lysis Buffer containing 2mg.ml Proteinase K and incubate O/N with shaking.
3. Sonicate 10" @ 100Watts, 2X. Place on ice between sonications.
4. Phenol:chloroform extract 1X.
5. Chloroform extract 1X.
6. NaOAc/EtOH precipitate 10' @ -70oC.
7. Resuspend in TE.
