D. ATP Synthesis

3 + ADP + Pi + H+ ---> 3 + ATP + H2O

Mitchell’s Chemiosmotic Hypothesis

 

 

Structural Biochemistry of the F0F1 ATP Synthase

 

 

 

The Binding-Change Mechanism

 

 
 

Binding Free Energy
     

Conformation

{ADP + Pi + H+}

{ATP + H2O}
     

L state

Negative

Positive

T state

Negative

Negative

O state

Positive

Positive

 

 

 

 

 

 

Transport of ATP Across the Inner Mitochondrial Membrane

 

 

Transport of NADH

 

2 + 2 ---> 2 + 2

 

 

 

 

 

Counting ATP

To generate one mole of ATP:

F0F1 SYnthase 3 moles of

Transport of ATP 1 mole of

Each Mole of NADH in Matrix Pumps 10 = 2.5 Moles ATP

Each Mole of NADH in Cytosol Pumps 6 = 1.5 Moles ATP

Each Mole of FADH2 in Matrix Pumps 6 = 1.5 Moles ATP

For 1 Mole of Glucose:

Glycolysis:

2 Moles of ATP

2 Moles of NADH in Cytosol = 3 Moles ATP

Total: 5 Moles of ATP

Pyruvate Dehydrogenase:

2 Moles of NADH in Matrix = 5 Moles ATP

Total: 5 Moles of ATP

TCA:

2 Moles GTP

6 Moles of NADH in Matrix = 15 Moles ATP

2 Moles of FADH2 in Matrix = 3 Moles ATP

Total: 20 Moles ATP

Inhibition, Regulation and Uncoupling of Ox. Phos.

Inhibitors and Regulation:

Oligomycin => ATP Synthase

Atractyloside => Adenine Nuc. Transporter

Bongkrekic Acid

 

Uncoupling:

Dinitrophenol (DNP) => Inner Mit. Membrane

Thermogenin (Uncoupling Protein)

Brown Adipose Tissue