Pathogenesis of Bacteria, Viruses and Eukaryotic Parasites

Microbiology and Immunology 210 (Winter 2003)

Monday and Wednesday 2:15-4:05pm, Friday 2:15-3:05pm, Fairchild Science Building D300

Peter Sarnow, Fairchild Science Building D309b, 8-7076

TAs: Mary Brinig and Marianne Campbell

BASIC BIOLOGY

1. Wed. Jan. 8 Introduction to bacterial pathogenicity (Falkow)

2. Fri. Jan.10 Introduction to fundamental virology: properties of genomes

and translation schemes (Sarnow)

3. Mon. Jan.13 Introduction to eukaryotic parasites: diversity in form and

function (Boothroyd)

ENTRY MECHANISMS

4. Wed. Jan.15 Uptake of viruses (Mocarski)

5. Fri. Jan.17 Microbial entry into host cells: invasion factors, integrin receptors (Relman)

6. Mon. Jan.20 Holiday

7. Wed. Jan.22 Invasion of host cells by eukaryotic parasites (Boothroyd)

GENE EXPRESSION

8. Fri. Jan.24 Bacterial gene expression in biofilms and in response to environment (Matin)

9. Mon. Jan.27 Regulation of bacterial virulence-associated genes and virulence-associated secretory and assembly pathways (Relman)

10. Wed. Jan.29 Gene expression in parasites: polycistronic transcription,

trans-splicing and RNA editing (Boothroyd)

11. Fri. Jan.31 Lytic infectious cycles (Sarnow)

12. Mon. Feb. 3 Latent viral infectious cycles (Mocarski)

13. Wed. Feb. 5 Retroviral gene expression (Nolan)

14. Fri. Feb. 7 Use of retroviruses in gene therapy (Blau)

HOST PATHOGEN INTERACTIONS

15. Mon. Feb. 10 Bacterial pathogen-plant cell interactions (Mudgett)

16. Wed. Feb. 12 Bacterial life within the vacuole and within the cytoplasm of the host cell (Theriot)

17. Fri. Feb. 14 Plasmodium-host interactions (Schneider)

18. Mon. Feb. 17 holiday

19. Wed. Feb.19 Subversion of host pathways by viruses (Sarnow)

20. Fri. Feb. 21 Pseudomonas-host interactions (Tan)

21. Mon. Feb. 24 Structural issues in host-pathogen interactions (Garcia)

22. Wed. Feb. 26 TA-lectures

23. Fri. Feb. 28 Recruitment weekend

EVOLUTION

24. Mon. Mar. 3 Viral evolution (Kirkegaard)

25. Wed. Mar. 5 Persistent bacterial infection (Falkow)

HOST IMMUNE RESPONSES

26. Fri. Mar. 7 Host immune response to viral infection-viral vaccines (Arvin)

27. Mon. Mar. 10 Antigentic variation and evolution in parasites (Boothroyd)

28. Wed. Mar.12 Host immune responses to parasites (Schneider)

29. Fri. Mar. 14 Antibiotic resistance in bacteria (Theriot)

EXAM WEEK

Reference Texts:

Fields, Virology and Fundamental Virology, 1995 editions. Raven Press

Salyers, Bacterial Pathogenesis - A molecular Approach, 1994. ASM Press.

Flint et al., Principles of Virology, 2000. ASM Press

Problem sets: A problem set will be distributed on February 5. This is an open book, take home exam which is due the following Wednesday. 30% of the final grade.

Final exam: Open book, take home exam. 70% of the final grade.

Final grade: 30% problem set, 70% final exam.