Post-Processing of Oligo Arrays on Corning Ultra GAPS slides
Post-Processing of Oligo Arrays on PolyLysine coated slides
Ambion MessageAmp II Amino Allyl and Cy Dye Coupling for Oligo Arrays (PDF Version) (updated 05/18/2006)
Preparation of Fluorescent DNA Probe from Total mRNA - Direct label of Total RNA for Oligo Arrays (PDF version) (updated 05/18/2006)
Array Hybridization and Washing for Oligo Arrays (PDF version)
Reverse Transcription (RT) and Amino-Allyl coupling of cDNA for (total) RNA sample input
Cy5 destruction has been reported after 10-30 seconds exposure to ozone levels above 5-10 ppb, especially during the array washing, drying and scanning steps. We have analyzed this problem and developed several solutions you can download here (updated June 2007):
Details on ozone destruction of Cy5 and plans for building excellent ozoone-free enclosurese.
A Good Cheap Kludge - how to build an ozone-free room.
How to dry microarray slides with an ozone-free room and small centrifuge.
of DNA Samples (updated 12/3/1999)
coating of slides (updated 10/6/1999)
aRNA Amplification Ambion method (updated 1/28/2003)
aRNA Amplification Wang protocol (updated 4/23/2002)
aRNA Amplification (updated 6/15/2001)
|Nucleic Acid Preparation|
Human FastTrack mRNA Isolation (updated 8/9/1999)
of total RNA from whole blood collected in PAXgene tubes (updated 6/18/2002)
|Nucleic Acid Labeling|
Transcription using Cy3- & Cy5-dUTP:
---Yeast (updated 3/29/1999)
---Human (updated 3/15/2001)
---E. coli (updated 2/22/1999)
Reverse Transcription and aa-UTP Labeling of RNA (updated 6/28/2000)
Revised amino-allyl labeling protocol (updated 2/3/2001)
Labeling Genomic DNA (updated 11/17/2000)
of Arrays (updated 9/19/1999)
Hybridization-General/Yeast (updated 8/10/1999)
Array Hybridization-Human (updated 3/15/2001)
dye HPLC purification
Design of Yeast Genomic Expression Experiments
Post questions on the Microarray Forum.